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. 2017 Apr 6;7:46002. doi: 10.1038/srep46002

Figure 2.

Figure 2

Diet-induced high Hcy upregulates 5LO enzymatic pathway via epigenetic modification. (A) Representative western blot analysis of 5LO protein in brain cortex homogenates from 3xTg mice receiving vehicle (Ctrl), folate and B vitamins deficient diet (Diet), Diet + zileuton, or zileuton. (B) Densitometric analysis of the immunoreactivity to the antibody shown in panel A. (C) Levels of LTB4 measured by a specific and sensitive ELISA assay in brain cortex homogenates from the same four groups of 3xTg mice. (D) Quantitative real time Reverse Transcription Polymerase Chain Reaction (q RT-PCR) analysis of 5LO mRNA in brain cortices of 3xTg mice receiving vehicle (Ctrl), folate and B vitamins deficient diet (Diet), Diet + zileuton, or zileuton. (E) 5LO DNA methylation levels in brain cortices of the 3xTg mice randomized to the same four treatment groups. (F) Representative western blot analyses for DNMT1, DNMT3α, DNMT3β in brain cortices of the same four groups of mice. (G) Densitometric analyses of the immuno-reactivity shown in the previous panel. Data presented are mean ± s.e.m. (*p < 0.05, n = 6). (n = 5 control, n = 6 Diet, n = 6 Diet + zileuton, n = 5 zileuton).