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. 2017 Apr 6;7:46002. doi: 10.1038/srep46002

Figure 3.

Figure 3

High Hcy-dependent increase in Aβ peptides levels and deposition is prevented by pharmacological blockade of 5LO. (A,B) RIPA-soluble (RIPA) and formic acid extractable (FA) Aβ1-40 and Aβ1-42 levels in brain cortex of 3xTg receiving vehicle (Ctrl), folate and B vitamin deficient diet (Diet), Diet + zileuton, or zileuton measured by sandwich ELISA (*p < 0.05). (C) Representative images of brains from the four groups of 3xTg mice immunostained with 4G8 antibody. (Scale bar: 500 μm). (D) Representative western blots of APP, ADAM-10, BACE-1, PS1, APH-1, Nicastrin, Pen-2, in brain cortex homogenates from 3xTg mice receiving vehicle (Ctrl), the diet (Diet), Diet plus zileuton, or zileuton. (E) Densitometric analyses of the immunoreactivities to the antibodies shown in the previous panel (*p < 0.05). Values represent mean ± s.e.m. (n = 5 control, n = 6 Diet, n = 6 Diet + zileuton, n = 5 zileuton).