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. 2017 Apr 6;7:46002. doi: 10.1038/srep46002

Figure 5.

Figure 5

Diet-induced high Hcy brain level affects synaptic integrity and neuroinflammation. (A) Representative western blot analyses of post-synaptic density protein 95 (PSD-95), synaptophysin (SYP), and microtubule-associated protein 2 (MAP2) in brain cortex homogenates of controls (ctrl), folate and B vitamins deficient diet (Diet), Diet + zileuton, or zileuton-treated 3xTg mice. (B) Densitometric analyses of the immunoreactivities to the antibodies shown in the previous panel (*p < 0.05). (C) Representative images of brain sections from 3xTg mice receiving vehicle (Ctrl), supplemented diet (Diet), Diet + zileuton, or zileuton alone immunostained with PDS-95, synaptophysin, and MAP2 antibodies (Scale bar: 100 μm) (D) Representative western blots of GFAP and CD45 in brain cortex homogenates from controls (Ctrl), Diet, Diet + zileuton, or zileuton-treated 3xTg mice. (E) Densitometric analyses of the immunoreactivities to the antibodies shown in the previous panel (*p < 0.05). Values represent mean ± s.e.m. (n = 5 control, n = 6 Diet, n = 6 Diet + zileuton, n = 5 zileuton).