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. 2017 Apr 6;7:45490. doi: 10.1038/srep45490

Figure 2. Differential accumulation of ROS and quantification of Na+ and K+ ions in B. juncea seedlings.

Figure 2

The 15 d old hydroponically grown seedlings were given different treatments such as control (1/2 MS), NaCl (125 mM), NaCl (125 mM) + TU (75 μM) and TU (75 μM). For NaCl + TU and TU alone treatments, 24 h pre-treatment with same concentration of TU was also given. The ROS accumulation in roots was checked at 4 h after treatment using DCF-DA staining (A). The root region was divided into three different zones and ROS intensity was quantified independently using Image J program (B). A total of 15 seedlings were stained for each treatment and experiment was repeated twice to check its reproducibility. The cations like Na+ (C) and K+ (D) were measured at 24 h after treatment in both root and shoot. The data represents the mean ± SE of three biological replicates. Different letters on bar graph have been put on the basis of LSD value derived from SPSS software (DMRT, P < 0.05). Small and capital case letter is used for root and shoot, respectively.