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. 2017 Feb 22;89(7):3853–3857. doi: 10.1021/acs.analchem.7b00043

Figure 1.

Figure 1

(A) Structure of 2′,7′-dichlorodihydrofluorescein diacetate (DCFH2-DA, nonfluorescent), its deacetylated derivative 2′,7′-dichlorodihydrofluorescein (DCFH2, nonfluorescent), and its oxidized end-product 2′,7′-dichlorofluorescein (DCF, fluorescent). (B) Standard operating procedure for the preparation and purification of DCFH2 (detailed in the Supporting Information, section S–VIII).