Table 2. Primers (rat) used in the experiment.
Studied gene | Sequence of oligonucleotides (5′-3′) | |
---|---|---|
TNF-α | F | CAAGAGCCCTTGCCCTAAGG |
R | CGGACTCCGTGATGTCTAAGTACTT | |
IL-1β | F | TCAGGAAGGCAGTGTCACTCA |
R | CATCATCCCACGAGTCACAGA | |
IL-6 | F | CTGATTGTATGAACAGCGATGATG |
R | GGTAGAAACGGAACTCCAGAAGAC | |
MCP-1 | F | GGCCTGTTGTTCACAGTTGCT |
R | CCTGCTGCTGGTGATTCTCTT | |
MIP-1α | F | GCCTGCTGCTTCTCCTATGG |
R | TTGGACCCAGGTCTCTTTGG | |
Wnt2b | F | CAGGAGTGGTCCATGCTATCAC |
R | AGCGGACACCATAGTGGATGT | |
Wnt4 | F | ACTGGACTCCCTGCCTGTCTT |
R | GTCCGGTCACAGCCACACTT | |
Wnt5b | F | GGGACCGTTTGAAAGAGAAGTATG |
R | TCATTTCGCAGGCAGTAGTCA | |
Wnt7b | F | TGCTTTGGCGTCCTCTACGT |
R | CTCCCCGATCACGATGATG | |
Wnt10a | F | TCCGACCTGGTCTACTTTGAGAA |
R | TCTGGCGCAGAATGTTGTGA | |
GAPDH | F | CGCATCTTCTTGTGCAGTG |
R | GAGGGTGCAGCGAACTTTATT |
F = forward; R = reverse.