Figure 5. LysM-Cre; p38α^f/f mice showed an increase in the size of monocyte pool.

(A) The number of the bone marrow Gr1^lowCD11b⁺ monocytes was increased in 6 month-old LysM-Cre; p38α^f/f mice compared to control mice. N = 3. (B) The number of the bone marrow Gr1^lowCD11b⁺ monocytes was increased in 2.5 month-old LysM-Cre; p38α^f/f mice compared to control mice, to a lesser extent than that of 6-month-oldmice. N = 3. (C) Western blot showed that p38 MAPK activation was enhanced in the bone marrow monocytes isolated from 6-month-old normal mice compared to that of 2.5-month-old mice. BM cells were flushed out of the bones and purified with the Percoll method at 4 ^oC, which were directly used for western blot analysis. Results from two pairs of mice were shown. For all results in Fig. 5, P-values are based on Student’s t-test. *p < 0.05, **p < 0.01 when the value of mutant mice was compared to that of control mice.