REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-GluAI (clone 4.9D)	Dong et al., 1997	N/A
Mouse anti-GluA2 (clone 15F1)	Eric Gouaux, Vollum Institute	N/A
Rabbit anti-GluA2/3 (JH4854)	Xia et al., 1999	N/A
Rabbit anti-GluA3 (JH4300)	Mao et al., 2010	N/A
Rabbit anti-GluN1 (JH2590)	Mao et al., 2010	N/A
Rabbit anti-GRASPI (JH2730)	Ye et al., 2000	N/A
Rabbit anti-GRIPI (JH2260)	Dong et al., 1997	N/A
Rabbit anti-PICKI (JH2906)	Xia et al., 1999	N/A
Rabbit anti-Syntaxin13	Synaptic Systems	Cat# 110 113, RRID: AB_10639254
Rabbit anti-EEA1	Cell Signaling Technology	Cat# 3288S, RRID: AB_2096811
Rabbit anti-LAMP1	Abcam	Cat# ab62562, RRID: AB_2134489
Mouse anti-GFP	Santa Cruz	Cat# sc-69779, RRID: AB_1123603
Chicken anti-GFP	Aves Labs	Cat# GFP-1020, RRID: AB_10000240
Rabbit anti-dsRed	Clontech	Cat# 632496, RRID: AB_10013483
Mouse anti-HA (clone 16B12)	Covance	Cat# MMS-101P, RRID: AB_2314672
Mouse anti-GRIP1	BD Biosciences	Cat# 611319, RRID: AB_398845
Mouse anti-Rab4	BD Biosciences	Cat# 610889, RRID: AB_398206
Mouse anti-PSD95	UC Davis/NIH NeuroMab	Cat# 75–028, RRID: AB_2307331
Mouse anti-mGluR5	Abcam	Cat# ab76316, RRID: AB_1523944
Mouse monoclonal anti-αTubulin (Clone B-5-1-2)	Sigma-Aldrich	Cat# T6074; RRID: AB_47758
Biological Samples
Human cell line BH0092 (GRASP1 _S73N)	Wu et al., 2007	European Collection of Authenticated Cell Culture (ECACC)
Human cell line 5897 (GRASP1 _R822Q)	Wu et al., 2007	Greenwood Genetic Center
Chemicals, Peptides, and Recombinant Proteins
Tetrodotoxin citrate	Tocris	Cat# 1069; CAS: 18660-81-6
Bicuculline methochloride	Abcam	Cat# ab120110; CAS: 53552-05-9
Glycine	Tocris	Cat# 0219; CAS: 5640–6
Strychnine hydrochloride	Abcam	Cat# ab120416; CAS: 1421-86-9
Picrotoxin	Abcam	Cat# ab120315; CAS: 124-87-8
AMPA	Tocris	Cat# 0254; CAS: 83643-88-3
DL-APV	Tocris	Cat# 0105; CAS: 76326-31-3
MNI-caged-L-glutamate	Tocris	Cat# 1490; CAS: 295325-62-1
Critical Commercial Assays
N/A	N/A	N/A
Deposited Data
N/A	N/A	N/A
Experimental Models: Cell Lines
Human: Human Embryonic Kidney (HEK) 293T cells	ATCC	Cat# CRL-3216
Experimental Models: Organisms/Strains
Mouse: Grasp1 KO in C57BL/6 background	This paper	N/A
Mouse: Thy1-GFP (line-M) in C57BL/6 background	Feng et al., 2000	N/A
Recombinant DNA
pEGFP-N1	Clontech	Cat# 632162
shGRASP1 (shRNA against GRASP1) on pEGFP backbone	This paper	N/A
HA:GRASP1 WT (from mouse) on pEGFP backbone	This paper	N/A
HA:GRASP1 S73N on pEGFP backbone	This paper	N/A
HA:GRASP1 R822Q on pEGFP backbone	This paper	N/A
pFUGW	Lien et al., 2008	Addgene plasmid# 37632
shGRASP1 on pFUGW backbone	This paper
HA:GRASP1 WT on pFUGW backbone	This paper	N/A
HA:GRASP1 S73N on pFUGW backbone	This paper	N/A
HA:GRASP1 R822Q on pFUGW backbone	This paper	N/A
GFP:Rab4	Gu et al., 2016	N/A
GRIP1 :myc	Thomas et al., 2012	N/A
SEP:GluA1	Lin et al., 2009	N/A
SEP:GluA2	Thomas et al., 2012	N/A
pDsRed2-N1	Clontech	Cat# 632406
Sequence-Based Reagents
shRNA targeting sequence: shGRASPI: 5′-GCTAAGCTCTCTGAGAAAT-3′	This paper	N/A
Primers for human GRASP1 mutation analysis, see Table S3	This paper	N/A
Software and Algorithms
MetaMorph	Molecular Devices	https://www.moleculardevices.com/systems/metamorph-research-imaging
Image J	NIH	https://imagej.nih.gov/ij/
ScanImage	HHMI	https://openwiki.janelia.org/wiki/display/ephus/ScanImage
Other
N/A	N/A	N/A