Table 2. VSBV-1 RNA levels in samples from squirrels collected in Germany and the Netherlands in 2015 that were positive for viral RNA and VSBV-1 antibodies*.

Animal and sample type	Country and holding no.
	Germany												The Netherlands
	I	I		I	II	II	III	IV	IV		IV		V	V
Species	Sciurus variegatoides							Callosciurus prevostii
Identity	48/15-1		48/15-2	48/15-11	49/15-1	49/15-6	75/15	122/15-1		122/15-2	133/15		3/16-1	3/16-2
Animal sex	M		F	M	M	F	M	F		M	M		F	M
EDTA blood/transudate	±		±	±	+	±	+	NA		NA	NA		±	±
Oral swab sample	+		±	++	+	+	+	+		+	+		NA	NA
Nose swab sample	±		±	+	+	±	+	+		±	±		NA	NA
Eye swab sample	+		±	+	+	±	+	NA		±	±		NA	NA
Palatine tonsil	++		++	++	++	+	++	++		++	++		+++	++
Mesenteric lymph nodes	++		++	+++	++	++	+	++		++	++		+	++
Brain	+++		+++	+++	+++	+++	+++	++		+++	+++		+++	+++
Brachial plexus	++		++	++	++	++	++	++		++	++		+	++
Medulla oblongata	+++		+++	+++	+++	+++	+++	++		++	++		+++	+++
Trigeminal ganglion	+		+	+++	+++	++	++	NA		++	++		++	+++
Kidney	++		+	++	+++	+++	++	++		++	++		++	+++
Salivary gland	++		+	+	++	++	++	++		++	++		±	+++
Urinary bladder	++		++	+++	+++	++	+	++		++	++		++	++
Lung	++		++	+++	+++	++	++	++		+	++		±	++
Heart	++		+	++	++	++	++	+		+	++		+	++
Spleen	+		±	++	++	+	++	++		++	++		±	+++
Liver	±		±	+	+	++	+	+		+	+		±	+
Pancreas	++		+++	++	++	++	++	++		++	++		+	++
Sex organ	+		+++	+	+++	+	+++	+++		++	++		++	+++
Skeletal muscles	+		±	+	++	+	++	++		++	++		+	++
Skin	++		+	++	++	++	++	++		++	++		+	++
Nose cross-section	++		++	+++	+++	++	+++	+		++	++		++	+++

*Data are represented in VSBV-1 genome equivalent copies per milliliter of template: +++, >10⁶; ++, 10⁴–10⁶; +, 10²–10⁴; ± 10⁰–10²). The VSBV-1–specific Mix 10 quantitative real- time PCR assay was used for quantification (limit of detection 1 genome equivalent/µL template). NA, sample not available; VSBV-1, variegated squirrel bornavirus 1.