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. 2017 Apr 6;7:46252. doi: 10.1038/srep46252

Figure 5. Effect of RA on NF-κB activation and expression of its relative gene products in DSS-induced colitis mice.

Figure 5

(A) The manifestation and translocation to the nucleus of p65 in colon tissues were observed by immunohistochemistry estimation. (B) Immunoscore of p65 in colon of DSS-induced mice was estimated. (C) Nuclear (N) and cytosol (C) extracts were prepared from colon tissues on 7 days of DSS administration and translocation of p65 to the nucleus and phosphorylation of IκB were estimated by western blot analysis using specific antibodies. C23 and α-tubulin were used as internal controls. (D) NF-κB-related proteins were determined by western blot analysis using specific antibodies. β-actin was used as internal controls. Relative ratio level was determined by densitometric analysis (Bio-rad Quantity One® Software) normalized to internal controls. Values are the mean ± SD (n = 10); ##P < 0.01, ###P < 0.001 vs control group; *P < 0.05, **P < 0.01, ***P < 0.001 vs the DSS-induced group; significances between treated groups were determined using ANOVA and Dunnett’s post hoc test.