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. 2017 Apr 6;7:46252. doi: 10.1038/srep46252

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Copyright © 2017, The Author(s)

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(A) The manifestation and translocation to the nucleus of pSTAT3 (Tyr705) in colon tissues were performed by immunohistochemistry. (B) pSTAT3 (Tyr705) immunoscore in DSS-induced mice was estimated. (C) Nuclear (N) and cytosol (C) extracts were prepared from colon tissues on 7 days of DSS administration and the phosphorylation and nuclear translocation of pSTAT3 were estimated by western blot analysis using specific antibodies. C23 and β-actin were used as internal controls. (D) The expression of STAT3 target protein was determined by western blot analysis using specific antibodies. Relative ratio level was determined by densitometric analysis (Bio-rad Quantity One^® Software) normalized to internal controls. Values are the mean ± SD (n = 10); ^##P < 0.01, ^###P < 0.001 vs control group; ^***P < 0.001 vs the DSS-induced group; significances between treated groups were determined using ANOVA and Dunnett’s post hoc test.