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. 2017 Apr 6;7:46246. doi: 10.1038/srep46246

Figure 2. OCT4 shRNA + Akti-1/2 suppresses the propagation of adherent cancer cells.

Figure 2

(A) U87, U251, NCCIT cells grown in 6-well plates were infected with lentiviruses harboring OCT4 shRNA (sh-OCT4) or culture medium only. 3 days post infection, cells were harvested and lysed, and the whole cell lysates were subjected to immunoblotting with an anti-OCT4. The amount of total NCCIT protein loaded was only 1/40 of that of U87 and U251 cells. (B) Adherent U87 cells were infected with sh-Scramble or sh-OCT4, and simultaneously treated with DMSO (vehicle) or 5 μM Akti-1/2 for 5 days. The images were captured under an Olympus IX81 microscope at 200X magnification. (C–E) Adherent U87 cells (C), U251 cells (D) or MCF7 cells (E) grown in 96-well plates were infected with sh-OCT4 and simultaneously treated with DMSO (vehicle) or 5 μM Akti-1/2, and subjected to MTT assay at each time point. Data were expressed as mean ± SD of triplicate measurements from one of three independent experiments which gave similar results. The colored asterisks indicate the difference between each treatment group and vehicle group by significance levels (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).