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. 2015 Dec 11;52(2):32–40. doi: 10.1016/j.jdsr.2015.09.001

Figure 4.

Figure 4

Effects of a ROCK inhibitor on ameloblasts. H–E staining of ROCK inhibitor (Y27632)-treated mouse ameloblasts shows disruption of polarity and enamel formation (A and B). Immunostaining for amelogenin and ameloblastin (C–F) shows that these proteins are no longer directionally secreted. Arrows indicate cells that do not express enamel matrix proteins. Arrowheads indicate cells that secrete enamel matrix proteins in all directions. Staining of F-actin, E-cadherin and β-catenin shows the polarized distribution of those molecules in differentiated ameloblasts (G, I and K arrows), whereas ROCK inhibition disrupts the polarized distribution (H, J and L). The figures were reproduced from the study by Otsu et al. [15]. am, ameloblasts; en, enamel; si, stratum intermedium.