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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Cell Biochem. 2016 Mar 16;117(10):2346–2356. doi: 10.1002/jcb.25532

Figure 4. Sef inhibits TGFβ/TNFα induced EMT in MCF-10A cells.

Figure 4

(A–E) RT-qPCR analysis of Sef and EMT marker expression in MCF-10A cells treated with 2.5ng/ml TGFβ plus 2.5 ng/ml TNFα, or 5 ng/ml TGFβ plus 5 ng/ml TNFα for 48h. (A) Sef, (B) Snail, (C) Slug, (D) N-cadherin, (E) E-cadherin (* indicates p<0.05 between NT and Sef shRNA transduced cells) (F) Immunoblot analysis of Sef and EMT markers of TGFβ/TNFα treated MCF-10A cells. (G) Morphologic changes by TGFβ/TNFα treatment and AdSefFL, AdSefIC, AdSefEC overexpression. (H) E-cadherin immunostaining (green) of TGF/TNFα treated and AdSefFL, AdSefIC, AdSefEC overexpression in MCF-10A cells. Cells were counterstained with DAPI (blue).