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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Cell Biochem. 2016 Mar 16;117(10):2346–2356. doi: 10.1002/jcb.25532

Figure 6. Overexpression of Sef in MDA-MB-231 reduces tumor size in an orthotopic xenograft model of breast cancer.

Figure 6

NOD/SCID mice were orthotopically engrafted with 2 ×106 MDA-MB-231 cells stably expressing either a control (pWZL) (n=4) or Sef (n=5). (A) Tumors were measured twice per week between control and Sef groups. (B) Photo of tumors extracted after 49 days of MDA-MB-231 cell implantation. (C) Tumor weight was measured after excision. (D) Real-time PCR of EMT marker gene expression in excised MDA-MB-231 control and Sef tumors. (E) Immunohistochemistry staining of active β-catenin, total β-catenin, Snail and Slug on control and Sef tumor sections.