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. Author manuscript; available in PMC: 2017 Apr 6.
Published in final edited form as: Nat Neurosci. 2016 Sep 19;19(12):1628–1635. doi: 10.1038/nn.4392

Figure 2. DMHLepR→ARC neurons are sufficient to inhibit homeostatic feeding.

Figure 2

(a–c), in vivo optogenetic stimulation of DMHLepR→ARC terminals (a) significantly reduced food consumption during the dark-cycle (b; n=12, repeated measures ANOVA, main effect of treatment (F(1,44)=171.10, p<0.0001), main effect of time (F(3,44)=48.48, p<0.0001) and interaction (F(3,44)=30.95, p<0.0001) and following an overnight fast (c; n=15, repeated measures ANOVA, main effect of treatment (F(1,84)=569.90, p<0.0001), main effect of time (F(3,84)=226.50, p<0.0001) and interaction (F(3,84)=43.74, p<0.0001). (d), photostimulation of DMHLepR→ARC terminals in a post-fast refeed paradigm was sufficient to inhibit food intake when applied before or after food consumption had begun (n=7 (off) and 6 (on), ANOVA, F(2,16)=6.73, p=0.0074). Abbreviations, Before, before food presentation; After, after the initiation of consumption. All data presented as mean±SEM; post-hoc p-values *p<0.05; ***p<0.001; ****P<0.0001.