Minocycline treatment decreased energy intake and body fat accumulation induced by HFD. Seven days exposure to HFD (High fat diet) significantly increased energy intake (A, p<0.001; n=8/group) and body fat accumulation (B, p<0.05; n=8/group). After 21 days, when compared to saline-treated animals, HFD minocycline-treated rats exhibited a significant decrease in energy intake (A, p<0.05; n=4/group), body fat accumulation (B, p<0.05; n=4/group) and in body weight (C, p<0.05; n=4/group). Minocycline reduced the caloric intake in LFD rats only in the first week (A). However, between the 8th and 21st day of the experiment, this effect was abolished (A). Minocycline did not affect body fat accumulation and body weight of LFD rats (B, C). Bars represent the average value ±SEM; a, b, c – different letters denote significant differences.