FIG 2.

Levels of parasitemia in individual Aotus monkeys immunized in a prime/boost regimen with plasmid DNA/Ad5 encoding P. vivax blood-stage antigens AMA1 and MSP1₄₂ and challenged with P. vivax SAL-1. (a) Group A, plasmid/virus control (n = 4); (b) group B, AMA1/AMA1 (n = 5); (c) group C, MSP1₄₂/MSP1₄₂ (n = 5); (d) group D, AMA1-MSP1₄₂/AMA1-MSP1₄₂ (n = 5); (e) group E, control/AMA1-MSP1₄₂ (n = 4); (f) group F, AMA1-MSP1₄₂/control (n = 4); (g) group G, infection control (n = 2). (h) Kaplan-Meier survival plot of animals not treated with MQ, color coded by group, to day 35 PoC. Red arrows, MQ rescue treatment for anemia (HCT less than 50% of that at the baseline); a, the animal was treated for anemia; T, the animal was treated to the end of the experiment on day 35 PoC; d, the animal died of causes unrelated to malaria. A level of parasitemia of 0.001 × 10³ parasites/μl was considered negative or undetectable by microscopy.