FIG 5.

Quantitative comparisons of antibodies directed to the infecting virus versus all other flaviviruses. Antibody recognition of microarrayed E proteins displayed as mean fluorescence intensity (±SEM). Antibodies from primary flavivirus infections of NHPs (ZIKV, DENV1 to DENV4, and YFV) and humans (rDEN2Δ30) exhibited significantly decreased recognition of heterologous E antigens compared to virus-specific E (dark gray) (P < 0.05 by one-way ANOVA with Tukey's range test). DENV E proteins are separated from all other flavivirus E proteins (including YFV, SLEV, POWV, TBEV, MVEV, WNV, JEV, and ROCV) to show DENV antibody cross-reactivity between serotypes.