Table 1. Screen of the probes a .
Probes | λ ex/λ em (nm) | DTT (F/F 0) | DTT + Msr A (F/F 0) |
1 | 395/505 | 11.2 | |
2 | 390/500 | 14.5 | |
3 | 385/505 | 216 | |
4 | 425/570 | 1.7 | 2.0 |
5 | 508/528 | 1.2 | 1.3 |
6 | 508/520 | 1.7 | 1.7 |
7 | 440/570 | 0.97 | 2 |
8 | 430/640 | 1.0 | 1.0 |
9 | 450/625 | 0.22 | 1.51 |
10 | — b | — b | — b |
11 | 390/550 | 1.92 | 2.32 |
12 | 410/550 | 0.81 | 0.53 |
13 | 370/497 | 1.32 | 1.47 |
14 | 375/550 | 10.7 | |
15 (Msr-blue) | 335/438 | 1.08 | 32.33 |
16 | 420/490 | 2.0 | 11.6 |
17 | 390/505 | 24.9 | |
18 | 375/480 | 1.0 | 1.1 |
19 | 380/580 | 1.0 | 1.0 |
20 | 400/510 | 0.37 | 2.38 |
21 | 340/490 | 0.57 | 1.42 |
22 | — b | — b | — b |
23 | 330/450 | 2.1 | 1.87 |
aThe assays were performed by incubating probes (10 μM) with DTT (5 mM) or DTT (5 mM)/Msr A (3 μg ml–1, 120 nM) for 1 h at 37 °C in TE buffer (50 mM Tris–HCl, 1 mM EDTA, pH 7.4).
bThere is only very weak fluorescence in the aqueous solution. All compounds were dissolved in DMF to prepare stock solutions, and the organic solvent in the final assay mixture is no more than 0.1% (v/v). The folds of fluorescence changes were expressed as F/F 0.