| Q1: What is average number of sweat tests per annum in your laboratory? |
10/10 |
| a) <50 |
2 |
| b) 50-100 |
3 |
| c) 100-300 |
3 |
| d) >300 |
3 |
| Q2: Where do you perform sweat stimulation and collection? |
10/10 |
| a) in a clinic |
4 |
| b) in a laboratory |
6 |
| Q3: What method do you use for sweat stimulation? |
10/10 |
| a) pilocarpine iontophoresis |
10 |
| b) wrapping a patient with plastic material |
0 |
| Q4: Who performs sweat stimulation and collection? |
10/10 |
| a) laboratory technician and/or technologist |
5 |
| b) medical biochemist |
1 |
| c) clinical staff |
4 |
| Q5: What type of information do you provide to parents or patients about sweat test? |
10/10 |
| a) only written |
0 |
| b) only verbal |
5 |
| c) verbal and written |
2 |
| d) by hospital web site |
1 |
| e) no information provided |
2 |
| Q6: Do you test new-borns less than 2 weeks of age? |
10/10 |
| a) yes |
2 |
| b) no |
8 |
| Q7: Do you test new-borns less than 3 kg of weight? |
10/10 |
| a) yes |
1 |
| b) no |
9 |
| Q8: Number of additional sweat tests carried out after first positive result? |
10/10 |
| a) 1 |
4 |
| b) 2 |
5 |
| c) > 3 |
0 |
| Q9: Which electrodes do you use for sweat stimulation? |
9/9 |
| a) stainless steel electrodes |
0 |
| b) cooper electrodes |
3 |
| c) electrodes of flexible size and curvature made by local manufacturer |
6 |
| d) electrodes of unspecified origin |
0 |
| Q10: How long do you collect sweat after pilocarpine iontophoresis? |
9/9 |
| a) < 30 min |
1 |
| b) 30 min |
8 |
| c) 30 - 40 min |
0 |
| d) > 40 min |
0 |
| Q11: What is the minimum acceptable sweat weight for chloride analysis? |
9/9 |
| a) 50 mg |
1 |
| b) 75 mg |
5 |
| c) 100 mg |
2 |
| d) no criteria for acceptable sweat weight |
1 |
| e) other, specify |
0 |
| Q12: What do you use for sweat collection? |
9/9 |
| a) pre-weighed gauze |
7 |
| b) pre-weighed filter paper |
2 |
| c) polypropylene or glass tube |
0 |
| Q13: What do you do when insufficient sweat weight is collected? |
9/10 |
| a) keep testing and carry out chloride analysis |
1 |
| b) stop testing and repeat sweat collection another time |
7 |
| c) carry out bilateral sweat collection |
1 |
| Q14: Do you use internal quality control? |
9/9 |
| a) yes, specify |
5 |
| b) no |
4 |
| Q15: What is the number of sweat test performed annually by the most involved individual staff? |
|
10/10 |
| a) ≤ 10 |
|
0 |
| b) 11 - 49 |
|
4 |
| c) ≥ 50 |
|
5 |
| d) no record |
|
1 |
| Q16: What are your laboratory reference ranges for sweat chloride in infants < 6 months of age? |
8/9 |
| a) negative < 30 mmol/L; intermediate 30 - 60 mmol/L; positive > 60 mmol/L |
3 |
| b) negative < 60 mmol/L; intermediate 60 - 80 mmol/L; positive > 80 mmol/L |
3 |
| c) other, specify |
2 |
| Q17: What are your laboratory reference ranges for sweat chloride in infants > 6 months of age and adults? |
9/9 |
| a) negative < 40 mmol/L; intermediate 40 - 60 mmol/L; positive > 60 mmol/L |
5 |
| b) negative < 60 mmol/L; intermediate 60 - 80 mmol/L; positive > 80 mmol/L |
1 |
| c) other, specify |
3 |
| Q18: Is collected sweat weight included in the laboratory sweat test report? |
9/9 |
| a) yes, specify |
3 |
| b) no |
6 |
| N – number of responders. TN – total number of participating laboratories. |
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