Figure 4.

IL-4, CCL11, CXCL10, and CXCL9 levels in whole lung samples from TLR3+/+ and TLR3−/− mice prior to and at days 8 and 16 after egg embolization. Whole lung lobes were homogenized in triton X-100 containing buffer and centrifuged to yield membrane-free supernatants, which were analyzed by ELISA (see Materials and Methods). Data shown are mean +/− SE from 1 representative of two separate experiments. Each time point had three-five mice per group. Significance was determined by using one-way ANOVA and Newman-Keuls post-test (* P ≤ 0.05,).