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Constitutive mRNA expression of TLRs in freshly isolated rat peritoneal mast cells. TLR2, TLR3, TLR4, TLR5, TLR7, and TLR9 mRNA expression were examined by qRT-PCR. Relative expression levels of TLR mRNAs, normalized to housekeeping gene β-actin. Each bar represents the mean ± SEM of four experiments performed
