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. 2017 Apr 6;7:45929. doi: 10.1038/srep45929

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Copyright © 2017, The Author(s)

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SDS-PAGE analysis of proteins (a–c) and western blotting of SlpA (d–f) in the wild type and the mutants. Compared with wild type cells, the 123 kDa SlpA (indicated by an arrow) was absent from the cell envelope and only its fragments were present in the peeling envelope fraction and whole cell envelope of ∆DR_146T. (a) Cell culture’s supernatant; (b) peeling cell envelope; (c) whole cell envelope separated from bacterial high pressure homogenate. (d) Whole cell extract; (e) peeling cell envelope of DR_146T; (f) whole cell envelope separated from bacterial high pressure homogenate. DRWT, D. radiodurans wild type; ∆DR_146T, mutant deficient in DR_146T; ∆DR_2577, mutant deficient in DR_2577; ∆DR_146T-DUF490, mutant deficient in the DUF490 motif of DR_146T. GroEL was used as a loading control.