Figure 6.

Effect of piperine on calpain activity. Differentiated THP‐1 macrophages were incubated for 24 h with or without (solvent vehicle control) piperine at 50 μM. Samples from cells were extracted and calpain activity was assessed as described in Section 2. An irreversible inhibitor of calpain (Z‐LLY‐FMK; 10 μM) and calpeptin (30 μg/mL) was used as positive control. All data are mean ± SD (n = 4) versus solvent vehicle control (DMSO), ***p < 0.001; n.s., no significance (one‐way ANOVA/Bonferroni).