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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Dev Biol. 2017 Feb 17;424(1):77–89. doi: 10.1016/j.ydbio.2017.02.007

Figure 3. Elf5 expression is down-regulated in mouse kidneys with Notch-signaling- deficient-collecting ducts.

Figure 3

A. Heatmap of genes with reduced expression in the kidneys of three mutant (mt) HoxB7->Cre; RBPJ−/f;Rosa+/EYFP E18.5 embryos compared with that of three wild type (wt) RBPJf/f ;Rosa+/EYFP E18.5 embryos. The genes listed were down-regulated in the mt kidneys by at least 1.25 fold, n=3 per genotype, p<0.05. The values in each row were normalized by subtracting the row mean and dividing by row standard deviation to calculate the z-scores. The heatmap color scale is −1.5 to 1.5 (z-score), with intensity of red correlating with increased level of expression and intensity of blue correlating with lower level of expression compared with mean value within each row, which is represented as white. The arrowheads point at known PC genes Aqp2 and Aqp4, while the arrow points at Elf5. B. Elf5, Aqp2 and Aqp4 expression levels are reduced, while that of Foxi1 and Atp6v1b1 are increased in E18.5 kidneys of HoxB7Cre; PS1−/f; PS2−/− (inactivation of presenilin1 (PS1) and presenilin2 (PS2) when compared with PS1f/f;PS2−/− littermates, n=4 per genotype and asterisks denote p<0.05, two-tailed unpaired t-test.