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. 2017 Apr 6;12(4):e0174940. doi: 10.1371/journal.pone.0174940

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© 2017 Urano-Morisawa et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 6 — RT-PCR analysis of NCDFCs exposed to osteoclast differentiation stimulating factors (M-CSF, RANKL) and an osteoclast differentiation inhibiting factor (OPG). Proliferative NCDFCs continuously expressed M-CSF. Addition of 1,25(OH)₂D₃ to the cultures suppressed the expression of OPG and induced production of RANKL by the cells.