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. 2017 Mar 31;12:2531–2551. doi: 10.2147/IJN.S129274

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© 2017 Ke et al. This work is published and licensed by Dove Medical Press Limited

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TRAIL combined with AuNPs promoted mitochondrial fission in NSCLC cells.

Notes: (A) Calu-1 cells were incubated with TRAIL and AuNPs, alone and together, for 6 h, and then mitochondrial morphology was detected by the staining of mito with MitoTracker^® Green. Typical confocal micrographs of mitochondrial morphology are shown. Magnification ×400. (B) Quantitative analysis of mitochondrial network was performed by using ImageJ software. At least 20 cells were analyzed per condition for each experiment. *P<0.05 compared to untreated groups. (C) Effects of TRAIL and AuNPs combination treatment on mitochondrial recruitment of Drp1 assessed by immunoblotting analysis. (D) Densitometric analysis was performed to evaluate the relative levels of mitochondrial and cytosolic Drp1 expression. *P<0.05, **P<0.01, compared to untreated groups. (E, F) Western blotting analysis was employed to detect the levels of mitochondrial fission/fusion proteins in cells after incubation with TRAIL and AuNPs, alone and together. The relative intensity of fission/fusion protein expression was quantified by densitometric analysis. β-actin and COX IV were used as cytoplasmic and mito markers, respectively. Results are shown as mean ± SD of three independent experiments. *P<0.05, compared to untreated groups.

Abbreviations: AuNPs, gold nanoparticles; cyto, cytoplasm; Drp1, dynamin-related protein 1; Fis1, fission protein 1; Mfn1, mitofusin 1; Mfn2, mitofusin 2; mito, mitochondria; NSCLC, non-small-cell lung cancer; Opa1, optic atrophy 1; p-Drp1, phosphorylation of Drp1; SD, standard deviation; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.

TRAIL combined with AuNPs promoted mitochondrial fission in NSCLC cells.

Notes: (A) Calu-1 cells were incubated with TRAIL and AuNPs, alone and together, for 6 h, and then mitochondrial morphology was detected by the staining of mito with MitoTracker^® Green. Typical confocal micrographs of mitochondrial morphology are shown. Magnification ×400. (B) Quantitative analysis of mitochondrial network was performed by using ImageJ software. At least 20 cells were analyzed per condition for each experiment. *P<0.05 compared to untreated groups. (C) Effects of TRAIL and AuNPs combination treatment on mitochondrial recruitment of Drp1 assessed by immunoblotting analysis. (D) Densitometric analysis was performed to evaluate the relative levels of mitochondrial and cytosolic Drp1 expression. *P<0.05, **P<0.01, compared to untreated groups. (E, F) Western blotting analysis was employed to detect the levels of mitochondrial fission/fusion proteins in cells after incubation with TRAIL and AuNPs, alone and together. The relative intensity of fission/fusion protein expression was quantified by densitometric analysis. β-actin and COX IV were used as cytoplasmic and mito markers, respectively. Results are shown as mean ± SD of three independent experiments. *P<0.05, compared to untreated groups.

Abbreviations: AuNPs, gold nanoparticles; cyto, cytoplasm; Drp1, dynamin-related protein 1; Fis1, fission protein 1; Mfn1, mitofusin 1; Mfn2, mitofusin 2; mito, mitochondria; NSCLC, non-small-cell lung cancer; Opa1, optic atrophy 1; p-Drp1, phosphorylation of Drp1; SD, standard deviation; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.