The size effect of AuNPs on mitochondrial morphology in NSCLC cells.
Notes: (A) After treatment with 15, 50, and 80 nm AuNPs for the indicated time points, Calu-1 were stained with MitoTracker® Green and changes in mitochondrial morphology were observed under a CLSM. Representative CLSM images are presented. (B) Quantitative analysis of mitochondrial morphology was carried out using ImageJ software. At least 20 cells were analyzed per condition for each experiment. *P<0.05, compared to the untreated control. (C, D) Immunoblot analysis of Drp1 in mitochondrial and cytosolic fractions at different time points after treatment with 50 nm AuNPs. (E, F) Immunofluorescence analysis of mitochondrial recruitment of Drp1 at 6 and 12 h post-treatment with AuNPs. The yellow color in the merged images represents the degree of colocalization between Drp1 (red) and mito (green). Magnification ×400.
Abbreviations: AuNPs, gold nanoparticles; CLSM, confocal laser scanning microscope; cyto, cytoplasm; Drp1, dynamin-related protein 1; mito, mitochondria; NSCLC, non-small-cell lung cancer; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.