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. 2017 Mar 31;12:2531–2551. doi: 10.2147/IJN.S129274

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© 2017 Ke et al. This work is published and licensed by Dove Medical Press Limited

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Drp1 silencing abrogated apoptotic and autophagic cell death induced by TRAIL and AuNPs combination treatment.

Notes: (A) Calu-1 cells were transfected with either a scrambled siRNA or an siRNA specific for Drp1, and Western blotting analysis was then carried out to confirm the knockdown efficiency of Drp1 knockdown. (B) Transfection alone did not affect cell viability of Calu-1 cells. (C) Drp1 silencing significantly abrogated the influence of combination treatment on poly (ADP-ribose) polymerase (PARP) cleaved and conversion of LC3-I to LC3-II. (D) The cell viability was estimated by the MTT assay in cells transfected with either a scrambled siRNA or a Drp1 siRNA after exposure to TRAIL and AuNPs, alone and together for 24 h. Values are mean ± SD (n=3). *P<0.05, compared to cells treated with scrambled siRNA and TRAIL.

Abbreviations: AuNPs, gold nanoparticles; Drp1, dynamin-related protein 1; MTT, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; SD, standard deviation; siRNA, small interfering RNA; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.