Fig 2. Probucol ameliorates oxidative stress and apoptosis in hyperoxic lung injury.
(A-D) The results of immunohistochemistry for 8-OHdG. (A) In room-air-exposed mice treated with probucol, expression of 8-OHdG was not detected. (B) In hyperoxia-exposed mice without probucol treatment, 8-OHdG was strongly expressed in nuclei of lung epithelial cells due to the tissue damage. (C) In hyperoxia-exposed mice treated with probucol, signal of 8-OHdG was decreased. (D) The immunostaining grade for 8-OHdG was significantly decreased by probucol treatment. Original magnifications: ×200. Data are shown as the mean ± SEM from four mice in each group. (E) Western blot analysis for p47phox. Each lane corresponds to the data from one mouse. (F) Relative band intensities from western blot analysis. Optical density values for each individual band were normalized to β-tubulin expression from the same tissue. Data are means ± SEM from three mice. **p < 0.01.