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. 2017 Apr 6;12(4):e0174973. doi: 10.1371/journal.pone.0174973

Fig 3. SHP-1 and FcγRIIB mediate impairment of IgG bead-phagosomes maturation by TDM.

Fig 3

DexTR-labelled WT untreated (A, B and C), SHP-1 siRNA, scramble siRNA-transfected (B) or FcγRIIB KO (C) BMDMs were either treated with 11 μM SHP-1 inhibitor (A) prior incubation with IgG or IgG TDM beads (ratio 1:5) or were directly incubated with beads (B and C) for 30 min. (A, B and C) The graphs indicate quantification by CLSM of the percentage of bead-phagosomes colocalising with dexTR. (B) 2 μg of protein extracts from SHP-1 siRNA or scramble siRNA-transfected BMDMs were assessed for SHP-1 expression by Western blot (loading control: β-actin). Data are expressed as means % of dexTR-positive bead-phagosomes and combined from two (A and B) and three (C) independent experiments done in triplicate ± interquartile ranges (Kruskal-Wallis test followed by Dunns post-test: *p<0.05; *** p<0.001). Western blot (C) is representative for two independent experiments.