Table 4. The changes in the levels of GlcN during the HCl hydrolysis of milk and standard GlcNAc.
Hydrolysis time (hr) |
3 hr/24 hr |
||||||
---|---|---|---|---|---|---|---|
GlcN concentration mg/100 g | 0 | 1 | 3 | 9 | 10 | 24 | ratio |
Elephant’s milk (Zuze) | 4 | 795 | 931 | 797 | 527 | 1.7 | |
Elephant’s milk (Pooly) | 966 | 572 | |||||
Cow’s milk mixture (Hosho) | 7 | 3 | 2.6 | ||||
Cow’s milk mixture (Toyo) | 7 | 3 | |||||
Mare’s milk (Yonaguni) | 20 | 31 | 29 | 19 | 1.6 | ||
Human milk (A) | 0 | 55 | 51 | 33 | 30 | 1.6 | |
Human milk (B) | 0 | 20 | 34 | 26 | 25 | ||
1,200 mg GlcNAc added to 100 g cow’s milk mixture (Urahoro) |
223 | 572 | 949 | 789 | 558 | 1.7 |
We monitored the time course of the GlcN levels from the start of hydrolysis (time 0) to 1, 3, 9, 10 and 24 hr. The maximum GlcN concentration was observed after approximately 3 hr hydrolysis, and the 3 hr: 24 hr GlcN level ratios ranged from 1.6 to 2.6. The 1,200 mg GlcNAc is equivalent to 1,000 mg GlcN.