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. Author manuscript; available in PMC: 2017 Oct 21.
Published in final edited form as: Anal Methods. 2016 Sep 15;8(39):7002–7013. doi: 10.1039/C6AY01947A

Figure 4.

Figure 4

Fully integrated microchip for multiplexed native Westerns. Reservoirs 1–8 provide access for sample, buffer and control voltages, which connect to a central assay chamber through a network of microfluidic channels. An expanded view of the central 1 mm × 1.5 mm microchamber is shown to the right. The patterned PA gel contains a sample loading zone, PAGE separation area, and two antibody-immobilized regions (Ab1 and Ab2) for immunoblotting. Proteins separated based on charge-to-mass in the PAGE region were electrotransferred to the antibody blotting area, where retention indicates binding to the immobilized probe. (Reprinted with permission from ref. 55. Copyright 2011 American Chemical Society.)