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Sequence of steps for WB analysis using the automated SimpleWestern method. The sample is loaded, stacked, and separated in a short capillary filled with sieving matrix. Photoactivation immobilizes the separated proteins to the capillary wall, which are then probed with primary antibodies. HRP-labeled secondary antibodies label the primary, leading to detection of the targeted protein. (Reprinted with permission from ref. 76. Copyright 2015 Humana Press.)
