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. 2017 Jan 9;7(1):e1271498. doi: 10.1080/21592799.2016.1271498

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Figure 5. — Effects of overexpression of GFP-Rab11-FIP1C wildtype and GFP-Rab11-FIP1C(S234A) on p(S234)-FIP1 abundance and localization. Parental T32 MDCK cells (A), and T23 MDCK cells expressing GFP-Rab11-FIP1C wildtype (B) or GFP-FIP1C(S234A) (C) were examined in non-switched cells (NS) and 6 hours after re-addition of calcium for immunostaining for p(S234)-Rab11-FIP1C and phalloidin staining. Overexpression of wildtype GFP-FIP1C and GFP-FIP1C(S234A) increased the lateral localization of pFIP1 staining in non-switched cells. Overexpression of GFP constructs did not inhibit phosphorylation of FIP1 by MARK2. X/Z images are shown below X/Y images, < indicates where images were taken for X/Y and X/Z planes. All scale bars = 10 µm.