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. 2017 Apr 6;12(4):e0175212. doi: 10.1371/journal.pone.0175212

Fig 3. E. faecalis RNase J2 activity is required for Ebp surface expression.

Fig 3

Flow cytometry analysis of (A) OG1RF with empty vector; (B) ΔrnjB with empty vector; (C) ΔrnjB expressing wild-type RNase J2; (D) ΔrnjB expressing inactive RNase J2 with His69 to Ala change; (E) ΔrnjB expressing inactive RNase J2 with His71 to Ala change; (F) ΔrnjB expressing active RNase J2 with Asp73 to Ala change. Cells grown in BHI medium with 25 ng/ml nisin to stationary phase were labeled with anti-EbpC mAb 69 followed by secondary phycoerythrin anti-mouse IgG conjugate.