Table 3. Effect of NaNO2 on some non-enzymatic parameters of oxidative stress in rat intestinal homogenates.
| Control | Dose of NaNO2 (per kg body weight) | ||||
|---|---|---|---|---|---|
| 20 mg | 40 mg | 60 mg | 75 mg | ||
| Total SH | 0.47 ± 0.03 | 0.43 ± 0.02 (-8.5) | 0.30 ± 0.02* (-36.2) | 0.27 ± 0.02* (-42.6) | 0.26 ± 0.02* (-44.7) |
| GSH | 8.0 ± 0.40 | 7.1 ± 0.40* (-11.3) | 6.3 ± 0.35* (-21.3) | 4.1 ± 0.21* (-48.8) | 3.6 ± 0.21* (-55) |
| LPO | 0.11 ± 0.006 | 0.18 ± 0.01* (+63.6) | 0.22 ± 0.01* (+100) | 0.25 ± 0.01* (+127) | 0.29 ± 0.02* (+163.6) |
| PO | 3.1 ± 0.15 | 3.9 ± 0.20* (+25.8) | 5.5 ± 0.30* (+77.4) | 6.8 ± 0.35* (+119.4) | 7.2 ± 0.38* (+132.3) |
| H2O2 | 0.24 ± 0.01 | 0.29 ± 0.02 (+21) | 0.35 ± 0.02* (+45.8) | 0.47 ± 0.03* (+95.8) | 0.52 ± 0.03* (+117) |
Total SH content is in μmoles/mg protein and GSH, LPO, PO and H2O2 are in nmoles/mg protein. Values in parenthesis represent percent change from control.
Results are mean ± standard error of six different preparations.
* Significantly different at p < 0.05 from control.
GSH, reduced glutathione; SH, sulfhydryl group; LPO, lipid peroxidation; PO, protein oxidation.