Table 2. Samples used to evaluate analytical specificity.
| Sample codes | Origins | Details | 16SrV statusa | Mean Ct valuesb |
|---|---|---|---|---|
| a | JKI Germany | Palatinate grapevine yellows 16SrV-C | 1 | 19.04 |
| b | DipSA USA | Aster yellows 16SrI-B | 0 | - |
| c | ANSES France | ‘Candidatus Phytoplasma solani’ 16SrXII-A | 0 | - |
| d | CRA-PAV Italy | Healthy grapevine | 0 | - |
| e | DipSA USA | ‘Candidatus Phytoplasma fraxini’ 16SrVII | 0 | - |
| f | DipSA Italy | Flavescence dorée phytoplasma FD-C | 1 | 27.97 |
| g | AGES Austria | Flavescence dorée phytoplasma FD-C | 1 | 24.69 |
| h | ANSES France | ‘Candidatus Phytoplasma solani’ 16SrXII-A | 0 | - |
| i | INRB Portugal | Flavescence dorée phytoplasma FD-D | 1 | 24.49 |
| j | DipSA Italy | Rubus Stunt phytoplasma 16SrV-E | 1 | 24.69 |
| k | ANSES France | Mix of healthy grapevine | 0 | - |
| l | NIB Slovenia | Flavescence dorée phytoplasma FD-D | 1 | 26.68 |
| m | ANSES France | Flavescence dorée phytoplasma | 1 | 25.05 |
| n | ANSES France | Flavescence dorée phytoplasma and ‘Candidatus Phytoplasma solani’ | 1 | 26.81 |
| o | ANSES France | Flavescence dorée phytoplasma | 1 | 28.35 |
| p | ACW Switzerland | Flavescence dorée phytoplasma | 1 | 23.58 |
| q | NIB Slovenia | Healthy grapevine | 0 | - |
| r | DipSA Italy | Grapevine yellows phytoplasma 16SrIII-B | 0 | - |
| s | DipSA China | Jujube witches’ broom phytoplasma 16SrV-B | 1 | 31.05 |
| t | ANSES France | Flavescence dorée phytoplasma | 1 | 26.10 |
| u | DipSA Europe | Elm witches’ broom phytoplasma 16SrV-A | 1 | - |
| v | ANSES France | Mix of healthy grapevine | 0 | - |
| w | IPEP Serbia | Flavescence dorée phytoplasma | 1 | 19.76 |
| x | ANSES France | Flavescence dorée phytoplasma | 1 | 31.95 |
a The 16SrV phytoplasma group is the target of each detection method because none of the evaluated methods is able to distinguish between FD phytoplasma and the other phytoplasmas of the same group
b Mean Ct value: 6 assays (except for sample “x”: 5 assays and sample “i": 4 assays).