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. 2017 Apr 6;12(4):e0175327. doi: 10.1371/journal.pone.0175327

Fig 3. Real-time PCR detection and quantitation of vRNA in CD4+, CD8+ and CD21+ leukocyte subsets enriched from blood and tissue compartments.

Fig 3

Provirus was detected in all leukocyte subsets from blood, MLN and splenic compartments. Viral RNA was undetectable (represented as ND for not detectable) in blood PBMC-derived CD4+, CD8+ and CD21+ leukocyte subsets harvested from all FIV-infected cats, except from CD4+ cells from cat 165. CD4+ leukocytes derived from MLN and spleen consistently contained detectable vRNA, while it was intermittently detectable in tissue derived CD21+ leukocytes and was rarely detectable in tissue derived CD8+ T cells. Bars represent the median. Student’s t-tests comparing the level of provirus or viral gag RNA between anatomical compartments among leukocytes subsets revealed no statistically significant differences between any tissues. All leukocytes subsets from all compartments in all cats revealed readily detectable GAPDH in both RNA and DNA fractions. Blue = cat 165, black = cat 184, green = cat 186, red = cat 187 (LTNP cat).