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. Author manuscript; available in PMC: 2017 Sep 29.
Published in final edited form as: Nature. 2017 Mar 29;544(7648):53–58. doi: 10.1038/nature21693

Extended Data Fig. 8. Irf8 and β-catenin function in self-renewing GMPs.

Extended Data Fig. 8

a, Representative FACS plots showing GMPs and Grs at steady state in Irf8+/+ and Irf8−/− mice. b, Experimental scheme for Irf8+/+ and Irf8−/− BM chimeric mice. c, Representative IF staining of donor-derived CD45.2+ (green) MP (red) in 5-FU-treated Irf8+/+ and Irf8−/− BM chimeric mice. d, Nuclear β-catenin in HSCs, MPP3 and MPP4 from Irf8+/+ and Irf8−/− mice. Results are expressed as percent positive cells. e, f, Experimental scheme for Ctnnb1 Ctrl and Ctnnb1 (e) loss-of-function (LOF) or (f) gain-of-function (GOF) mice. Stars indicate pGMPs and dotted lines cGMPs. Results are expressed as mean ± S.D.