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. Author manuscript; available in PMC: 2017 Sep 20.
Published in final edited form as: Nature. 2017 Mar 20;544(7648):88–91. doi: 10.1038/nature21679

Figure 3. Neural tube ablation disrupts ENS development.

Figure 3

a–c, Whole-mount images of T30 lamprey embryos (Red:HuC/D and acetylated tubulin; Cyan:5HT). a, Control nonablated embryo. b, Ablation of the neural tube at T24 results in a decrease of serotonergic (5HT+) cells in the adjacent gut relative to an equivalently sized anterior region. c, A sham-ablated embryo, in which epidermis and neural tube were cut but not removed, does not show a reduction of cells. d, Ablated embryos show a significantly lower ratio of serotonergic cells (# cells in surgical zone/# cells in anterior zone) than sham-ablated controls (Ttest; P=0.0004) and non-ablated batch controls (Ttest, P=0.0176). Error bars indicate s.e.m. e, Schematic model of neural crest contributions to the ENS in mouse compared with lamprey. Vagal neural crest (V; purple); Schwann cell precursors (SCP; blue); Lamprey branchial crest (BC; purple). Scale bar indicates 100 um.