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. 2017 Feb 16;25(4):989–1002. doi: 10.1016/j.ymthe.2017.01.010

Figure 4.

Figure 4

rOMV Source Strain Dependence on Morbidity, Immune Response, and Mortality

(A and B) Mice were weighed daily for 1 week post-prime (A) and boost (B) immunization. Analyzed using ANOVA followed by multiple comparisons using Dunnett method of correction. Error bars represent SD of mean (*p < 0.001). (C and D) Total IgG (C) and IgG isotypes IgG1 and IgG2a (D) anti-M2e titers of BALB/c mice 8 weeks post-prime rOMV immunization. Dotted line indicates lowest titer detectable above background (serum from PBS-vaccinated mice). Log-transformed IgG1 and IgG2a anti-M2e titers compared using paired t test. Error bars indicate 95% CI of geometric mean (*p < 0.05) (n = 11 CC rOMV-vaccinated mice, n = 12 Nsl rOMV-vaccinated mice, n = 16 PBS-vaccinated mice). (E and F) Mortality (E) and weight loss (F) of mice challenged with a lethal dose (50 FFU) of influenza A/PR8 (n = 5 CC rOMV vaccinated, n = 5 Nsl rOMV vaccinated, n = 5 PBS vaccinated, n = 5 pre-exposed). Kaplan-Meier survival curves were analyzed with a log-rank test using the Bonferroni method to account for multiple comparisons. Error bars on morbidity curves represent SEM.