Figure 5.

Immune Response, Mortality, and Morbidity in C57BL/6 and DBA/2J Mice

(A) Total IgG and isotypes IgG1 and IgG2c titers of C57BL/6 mice 8 weeks post-prime rOMV vaccination. Dotted line indicates lowest titer detectable above background. Log-transformed IgG1 and IgG2c titers compared using paired t test. Error bars indicate 95% CI of geometric mean (n = 10 CC rOMV-vaccinated mice) (*p < 0.0001). (B and C) Mortality (B) and weight loss (C) of mice challenged with a lethal dose (100 FFU) of influenza A/PR8 (n = 5 CC rOMV vaccinated, n = 5 PBS vaccinated, n = 5 pre-exposed). Kaplan-Meier survival curves were analyzed with a log-rank test. Error bars on morbidity curves represent SEM. (D) Total IgG and isotypes IgG1 and IgG2a titers of DBA/2J mice 8 weeks post-prime rOMV vaccination. Dotted line indicates lowest titer detectable above background. Log-transformed IgG1 and IgG2c titers compared using paired t test. Error bars indicate 95% CI of geometric mean (n = 5 CC rOMV-vaccinated mice) (*p < 0.0001). (E and F) Mortality (E) and morbidity (F) of DBA/2J mice challenged with a lethal dose (5,000 PFU, ∼2.5 × LD₅₀) of influenza A/X-47 (n = 5 CC rOMV vaccinated, n = 5 PBS vaccinated). Kaplan-Meier survival curves were analyzed with a log-rank test. Error bars on morbidity curves represent SEM.