Figure 1.

In Vivo CTL Assay

C57BL/6 mice were immunized subcutaneously once with KALA-OVA-LPs, R8-OVA-LPs, OVA-LPs (DOPE or EPC as a helper lipid), or free OVA protein at a dose of 3.1, 13, 25 (KALA-OVA-LPs only), or 50 μg OVA. Fluorescent-labeled target cells (OVA_257–264 pulsed, CFSE^High) and control cells (no peptide pulsed, CFSE^Low) were injected intravenously at 1 week after immunization. The OVA-specific lysis was calculated from the target cell/control cell ratio measured by flow cytometer 20 hr after injection. Data are mean + SEM (n = 3–5).