Figure 5.

Antigen Presentation Activity in the Presence of Chloroquine, an Endosome-Disruptive Agent

BMDCs were pre-incubated with 0, 25, and 50 μM chloroquine in medium, followed by treatment of the KALA-OVA-LPs or the R8-OVA-LPs at a lipid dose of 32 μM. After 5 hr, the treated cells were co-cultured with a B3Z T cell hybridoma for 15 hr at 37°C. The co-cultured cells were lysed and incubated with chrolophenol red β-D-galactopyranoside buffer for 4 hr at 37°C. The absorbance at 595 nm was used as an index for antigen presentation activity. Data are mean + SD (n = 6).