Fig. 2.

Reduced circulating NKT cell numbers in the peripheral blood multiple trauma patients. Freshly isolated PBMC from 22 HCs and 14 patients with multiple trauma were stained with FITC-conjugated anti CD3, PerCP-conjugated anti-CD4, APC-conjugated anti-CD8α, and PE-conjugated 6B11 mAbs, and then analyzed by flow cytometry. Percentages of NKT cells were calculated within a lymphoid gate. (A) Representative NKT cell percentages as determined by flow cytometry. (B) NKIT cell percentages among peripheral blood lymphocytes. (C) Absolute NKT cell numbers (per microliter of peripheral blood). Symbols (●) represent individual subjects and horizontal lines are median values.

NKT = natural killer T, PBMC = peripheral blood mononuclear cell, HCs = healthy controls, FITC = fluorescein isothiocyanate, APC = allophycocyanin, PE = phycoerythrin, mAbs = monoclonal antibodies, ANCOVA = analysis of covariance.

^*P < 0.05, ^†P < 0.01 by ANCOVA test.