Figure 3.

Characterization of Mouse WT1-Specific IFN-γ Responses and Immunodominant Epitopes for pSynConWT1-S Vaccine

(A) DNA vaccine immunization schedule showing the dosage of vaccine. C57BL/6 (B6) mice (n = 5 per group) were immunized at weeks 0, 2, 4, and 6 with pSynConWT1-S, pMuWT1-opt, and pMuWT1-nonopt via i.m. injection or EP. (B) Frequency of WT1-specific IFN-γ spot-forming units (SFUs) per million splenocytes isolated from vaccinated mice, determined by IFN-γ ELISpot assay using mouse peptides. (C) Matrix mapping to determine the WT1 mouse-specific immunodominant epitopes, comparing naive and pSynConWT1-S vaccinated mice. (D) List of immunodominant epitopes identified in the matrix mapping in (C), and comparison to the corresponding SynCon sequence. Error bars represent the average ± SEM.