Figure 6.

Early Treatment with PD-1 or TIM-3 Blocking Antibody Therapy Does Not Alter Late-Stage Memory Phenotypes

(A) Surviving mice from the experiment in Figure 3C were re-challenged with i.v. B16-OVA on day 193. A submandibular vein bleed was taken 1 day before re-challenge and the mice were sacrificed 1 week after re-challenge. (B and C) Frequencies of effector (CD44^hiCD62L^lo) or central memory (CD44^hiCD62L^hi) cells gated on CD8⁺ cells. (D) Gating strategy to identify SIINFEKL tetramer⁺ CD8⁺ cells and effector or central memory phenotypes. (E and F) Frequency of SIINFEKL tetramer⁺ cells gated on CD8⁺ cells and effector cells gated on CD8⁺ tetramer⁺ cells. Each of the points represents 3–5 mice/group. The median and interquartile range are shown.