Figure 3.

Workflow to study the genomic content of bacteriophages. Genomics of phages initiates by filtering phage particles from microbial community (A) through 0.22 μ filter which results in (B) virus particles containing residual DNA and RNA of other microbial communities. (C) Purification of virus particles from residual DNA and RNA are removed by CsCl density gradient method. (D) Phage particles are concentrated using polyethylene glycol or ultra-centrifugation. (E) Extraction of viral DNA uses kits or conventional methods. The DNA is amplified and libraries are prepared and subsequently sequenced them.